RBS
SpaCBA RBS

Part:BBa_K2760006:Design

Designed by: Fernanda Diaz   Group: iGEM18_TecMonterrey_GDL   (2018-10-09)


RBS for Lactobacillus rhamnosus GG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

    • Designed and optimized for L. rhamnosus GG.


Source

This ribosome binding site sequence was taken from the spaCBA pilus operon promoter region. From the coding region of the spaC pilus gene in the L. rhamnosus GG. The whole region for the ORF minus the promoter was taken [1].

References

[1] Rintahaka, Johanna & Yu, Xia & Kant, Ravi & Palva, Airi & von Ossowski, Ingemar. (2014). Phenotypical Analysis of the Lactobacillus rhamnosus GG Fimbrial spaFED Operon: Surface Expression and Functional Characterization of Recombinant SpaFED Pili in Lactococcus lactis. PloS one. 9. e113922. 10.1371/journal.pone.0113922.